Back to article: The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow


FIGURE 5: Shear-stress-induced autophagy and cell size regulation are abolished in FLCN-null cells. UOK 257 FLCN-null cells and UOK 257-2 FLCN restored cells were cultured on microslides and then subjected to fluid flow for 4 days (shear) or not (static 4D). Cells were fixed, labeled with DAPI, immunostained for LC3 (A, C) or labeled with DAPI and immunostained for β-catenin to reveal cell boarder (E, G) and then analyzed by fluorescence microscopy. (B, D) LC3 dots were quantified from experiments shown in (A) (UOK 257cells) and (C) (UOK 257-2 cells). (F, H) cells areas were quantified from experiments shown in (E) (UOK 257cells) and (G) (UOK 257-2 cells). Scale bars in (A, C, E and F) = 10μm.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close