FIGURE 6: Rnh1 interacts with RPA. Co-immunoprecipitation experiments were performed in triplicate. HA-Rnh1 has been overexpressed by galactose addition and immunoprecipitated with an anti-HA antibody. The presence of RPA in the HA-Rnh1 IP is monitored with an anti-Rfa1 antibody. Cell extracts treated with nuclease (Thermofisher) are indicated (+). Yeast strains were grown in medium containing 2% glucose (-) or 2% galactose (+) when mentioned. Total proteins on the membrane were stained with Ponceau S as a loading control (Bottom). MW: molecular weight.