FIGURE 2: Neuronal knockdown of Lamin causes an age-dependent loss of PAM cluster dopaminergic neurons. (A) Schematic of the posterior dopaminergic neurons of the Drosophila brain. Representative images of posterior dopaminergic neuron clusters in (B) elav/+ and (C) elav/Lam IR flies at 50 days old. (D) Schematic of the anterior dopaminergic neurons of the Drosophila brain. Representative images of the anterior dopaminergic neuron clusters in (E) elav/+ and (F) elav/Lam IR flies at 50 days old. G Quantification of dopaminergic neuron number in the posterior and anterior clusters of the fly brain at 50 days. (H) Quantification of dopaminergic neurons within the PAM cluster of the fly brain. n³7 animals per group. Data represents mean ± SEM and was analysed using student’s t-test. *p<0.05; **p<0.01; ***p<0.001. elav/+ = elavGal4; +/+; UAS-mCD8GFP > w¹¹¹⁸; elav/Lam IR = elavGal4; +/+; UAS-mCD8GFP > Lam IR.