FIGURE 2: Muscle-specific knockout of p38b MAPK abrogates activation of autophagy, UPP and muscle wasting in tumor-bearing mice. Mice with muscle-specific knockout of p38β MAPK and control mice (p38β MAPK^f/f) were implanted with LLC cells. In 21 days, mice were euthanized for analysis of muscle wasting. Lysate of TA collected from the mice was analyzed by Western blotting for LC3 and p62 to evaluate autophagy activity (A) and UBR2, atrogin1 and MHC to evaluate UPP activity (B). Muscle wasting in the mice was analyzed by examin-ing tumor weight, body and muscle weight, tyrosine release and grip strength (C). Muscle mass was measured as muscle fiber cross sectional area (D). Data in A to C were analyzed by one way ANOVA. Data in D were analyzed by Chi-square analysis. * denotes a difference (p < 0.05).