FIGURE 4: Effect of miR-200c overexpression in fibroblasts on NFκB and HIF pathways. (A) Phospho-NFκB p65 expression of fibroblasts with miR-200c downregulation or overexpression. (B) NFκB activity in NIH3T3 NFκB luciferase reporter cells with miR-200c overexpression or downregulation under normoxic (21% O2) and hypoxic (1% O2) conditions (Random Luciferase Units-RLU). *p<0.05. (C) Cytokine, chemokine and receptor expression profile in fibroblast conditioned media. BJ1 F200c or control were cultured in serum-free DMEM media. The levels of various factors in the cell-free culture media were measured by antibody arrays at day 2 and 4. Data was showed as fold change ± SD. *p<0.05. (D) HIF and MCT4 expression in fibroblasts with miR-200c modulation. (E) HIF activity in NIH3T3 HIF luciferase reporter cells with miR-200c overexpression or downregulation under normoxic and hypoxic conditions. *p<0.05. (F) Lactate level in BJ1miR-200c and control under normoxic and hypoxic conditions. *p<0.05.