FIGURE 3: Epigenetic state of the H19 locus in human HCC tissues (tumor) and non-tumorous tissues (non-tumor). (A) LOI was analyzed by RFLP analysis of 9 informative gDNA samples. Representative agarose gel with gDNA and cDNA before (left) and after digestion with the restriction enzyme AluI (right). (B) H19 promoter methylation represented as fractional β-values from GEO dataset GSE57956 (each, n=58, Kolmogorov-Smirnov test).